The northern maidenhair reproduces mainly by spores. These give rise to the male and female gametes necessary for the species to reproduce.
The sori (which contain the spores) are located along the margins of the fronds, on the underside of the segments, along the indented edge. They develop in spring and early summer, from May to July.
Once the spores have germinated and fertilization is complete, small green flat structures appear on the underside of the leaves. These structures are called prothalli. They emerge about two weeks after fertilization.
After the prothalli have formed, it takes more than 20 weeks for young fronds to appear.
Northern maidenhair also reproduce through its creeping rhizome, which is usually blackish, like the stalks, and extends horizontally along the ground. When conditions are favourable, this fern species forms dense colonies.